I feel your pain. We have around 80 acres in SC. Been in the family for years. One of the borders is a railroad, and another is a major hwy. Over the past few years, trespassing has become a huge problem. Most of them, when confronted, have left without issue.
However, one guy preceded to tell us he's been hunting for years, and we can't stop him. After going to court, he ended up being fined around $500. He said he's still going to hunt and fish, because nowhere in the state, can you hunt and fish for $500. This guy is a real piece of work. We have posted signs, fences, gates etc. But most come from walking from off of the RR tracks.
We did find a trail where someone had made coming from the hwy, and subsequently cut down several large trees to block it off and that has fixed most of the problem, so if possible, I'd start by making the trail look less like a trail. YMMV
However, one guy preceded to tell us he's been hunting for years, and we can't stop him. After going to court, he ended up being fined around $500. He said he's still going to hunt and fish, because nowhere in the state, can you hunt and fish for $500. This guy is a real piece of work. We have posted signs, fences, gates etc. But most come from walking from off of the RR tracks.
We did find a trail where someone had made coming from the hwy, and subsequently cut down several large trees to block it off and that has fixed most of the problem, so if possible, I'd start by making the trail look less like a trail. YMMV